MOLECULAR BIOLOGY (Code: ZLG0600204)

 

MOLECULAR BIOLOGY

Unit 1:

Nucleic Acids: Structure and types of DNA and RNA, Watson and Crick model of DNA

DNA Replication: Enzymes used in DNA Replication, DNA Replication in prokaryotes and eukaryotes, mechanism of DNA replication, Semi conservative, bidirectional and semi-discontinuous replication, Telomere and replication of telomeres

Unit 2:

Transcription: RNA polymerase structure and transcriptional Unit, mechanism of transcription in prokaryotes and eukaryotes

Post Transcriptional Modifications and Processing of Eukaryotic RNA: Split genes: concept of introns and exons, splicing mechanism and alternative splicing

Translation: Genetic code, Degeneracy of the genetic code and Wobble Hypothesis; aminoacyl tRNA synthetases and charging of tRNA; Proteins involved in initiation, elongation and termination of polypeptide chain; Mechanism of translation, Inhibitors of protein synthesis

Unit 3:

Regulation of gene expression: Operon concept, Transcription regulation in prokaryotes (lac operon and tryptophan operon)

Transcription regulation in eukaryotes: Activators, repressors, enhancers, silencer elements; Gene silencing and Genetic imprinting

DNA Damage and Repair Mechanisms

RNA interference

Practical

1. Study of Polytene chromosomes from Chironomous / Drosophila larvae

2. Preparation of metaphase chromosome from the bone marrow of mice

3. Quantitative estimation DNA using colorimeter (Diphenylamine

reagent)

4. Quantitative estimation of RNA using Orcinol reaction

5. Isolation of DNA from tissues and qualitative analysis by agarose gel electrophoresis

6. Study and interpretation of electron micrographs/ photograph showing: DNA replication, Transcription and Split genes