MOLECULAR BIOLOGY (Code: ZLG0600204)

 

Unit 1:

MOLECULAR BIOLOGY

Nucleic Acids: Structure and Types of DNA and RNA, Watson and Crick model

of DNA.

DNA Replication: Enzymes used in DNA Replication, DNA Replication in

prokaryotes and eukaryotes, mechanism of DNA replication, Semi

conservative, bidirectional and semi-discontinuous replication,

Telomere and replication of telomeres

Unit 2:

Transcription: RNA polymerase structure and transcriptional Unit, mechanism

of transcription in prokaryotes and eukaryotes

Post Transcriptional Modifications and Processing of Eukaryotic RNA: Split

genes: concept of introns and exons, splicing mechanism and alternative

splicing

Translation: Genetic code, Degeneracy of the genetic code and Wobble

Hypothesis; aminoacyl tRNA synthetases and charging of tRNA; Proteins

involved in initiation, elongation and termination of polypeptide chain;

Mechanism of translation, Inhibitors of protein synthesis

Unit 3:

Regulation of gene expression: Operon concept, Transcription regulation in

prokaryotes (lac operon and tryptophan operon)

Transcription regulation in eukaryotes: Activators, repressors, enhancers,

silencer elements; Gene silencing and Genetic imprinting.

DNA Damage and Repair Mechanisms

RNA interference

Practical

1. Study of Polytene chromosomes from Chironomous / Drosophila larvae

2. Preparation of metaphase chromosome from the bone marrow of mice

3. Quantitative estimation DNA using colorimeter (Diphenylamine

reagent)

4. Quantitative estimation of RNA using Orcinol reaction

5. Isolation of DNA from tissues and qualitative analysis by agarose gel

electrophoresis.

6. Study and interpretation of electron micrographs/ photograph showing:

DNA replication, Transcription and Split genes